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chmp6 egfp  (Addgene inc)


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    Structured Review

    Addgene inc chmp6 egfp
    Chmp6 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chmp6+egfp/CHMP6_GFP+(Plasmid+%2331806)/pmc09123275-233-10-11
    Average 93 stars, based on 6 article reviews
    chmp6 egfp - by Bioz Stars, 2026-09
    93/100 stars

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    Transfection:

    Article Title: Cellular abundance of sodium phosphate cotransporter SLC20A1/PiT1 and phosphate uptake are controlled post-transcriptionally by ESCRT
    Article Snippet: .. For CHMP6-EGFP transfection experiments, HEK293T cells were transiently transfected with CHMP6-EGFP (Addgene; catalog no.: 31806; gift from Daniel Gerlich) using FugeneHD transfection reagent (Promega; catalog no.: E2311), grown for 24 h, fixated after aspiration of incubation medium, permeabilized, blocked, and stained with SLC20A1 antibody (Cell Signaling; catalog no.: 12765) followed by Alexa 594–conjugated secondary antibody (Cell Signaling; catalog no.: 8889). .. Integrity of the CHMP6-EGFP construct was confirmed using Sanger sequencing (UT Southwestern McDermott Center Sanger Sequencing Core) and immunoblot for CHMP6 (Abcam; catalog no.: ab235050) and EGFP (Abcam; catalog no.: ab290) (data not shown).

    Incubation:

    Article Title: Cellular abundance of sodium phosphate cotransporter SLC20A1/PiT1 and phosphate uptake are controlled post-transcriptionally by ESCRT
    Article Snippet: .. For CHMP6-EGFP transfection experiments, HEK293T cells were transiently transfected with CHMP6-EGFP (Addgene; catalog no.: 31806; gift from Daniel Gerlich) using FugeneHD transfection reagent (Promega; catalog no.: E2311), grown for 24 h, fixated after aspiration of incubation medium, permeabilized, blocked, and stained with SLC20A1 antibody (Cell Signaling; catalog no.: 12765) followed by Alexa 594–conjugated secondary antibody (Cell Signaling; catalog no.: 8889). .. Integrity of the CHMP6-EGFP construct was confirmed using Sanger sequencing (UT Southwestern McDermott Center Sanger Sequencing Core) and immunoblot for CHMP6 (Abcam; catalog no.: ab235050) and EGFP (Abcam; catalog no.: ab290) (data not shown).

    Staining:

    Article Title: Cellular abundance of sodium phosphate cotransporter SLC20A1/PiT1 and phosphate uptake are controlled post-transcriptionally by ESCRT
    Article Snippet: .. For CHMP6-EGFP transfection experiments, HEK293T cells were transiently transfected with CHMP6-EGFP (Addgene; catalog no.: 31806; gift from Daniel Gerlich) using FugeneHD transfection reagent (Promega; catalog no.: E2311), grown for 24 h, fixated after aspiration of incubation medium, permeabilized, blocked, and stained with SLC20A1 antibody (Cell Signaling; catalog no.: 12765) followed by Alexa 594–conjugated secondary antibody (Cell Signaling; catalog no.: 8889). .. Integrity of the CHMP6-EGFP construct was confirmed using Sanger sequencing (UT Southwestern McDermott Center Sanger Sequencing Core) and immunoblot for CHMP6 (Abcam; catalog no.: ab235050) and EGFP (Abcam; catalog no.: ab290) (data not shown).



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